Department of Applied Biochemistry

DEPARTMENT OF APPLIED BIOCHEMISTRY

 

FIRST YEAR: FIRST SEMESTER

CODECOURSE TITLECREDIT UNIT
MAT 101Elementary Mathematics3
GSS 101Use of English I1
CSC 101Intro. Comp. Programming3
PHY 101General physics I3
BIO 101General Biology I3
GSS 105Humanities2
GSS108Igbo basic studies1
 TOTAL16

 

FIRST YEAR: SECOND SEMESTER

CODECOURSE TITLECREDIT UNIT
MAT 102Elementary Mathematics I3
GSS 102Use of English II1
CSC 102Intro. to Computer Prog. II3
PHY 102General physics II3
BIO 102General biology II3
GSS 106Social sciences2
GSS 109Igbo basic studies II1
 TOTAL16

 

YEAR TWO: FIRST SEMESTER

CODECOURSE  TITLECREDIT UNIT
BUS 101Intro. to Business I1
GSS 107Nigerian peoples and culture2
ICH 101Basic organic Chemistry2
ICH 111Basic inorganic chemistry2
MAT 201Linear Algebra3
PHY 201Modern physics3
 TOTAL13

 

YEAR TWO: SECOND SEMESTER

CODECOURSE  TITLECREDIT UNIT
ICH 202Basic physical chemistry2
BCH 102Intro. Biochemistry3
MAT 202Elem. Diff. Equations3
STA 202Intro. to statistical inference3
BUS 102Intro. to Business II1
ICH 112Practical chemistry2
GSS 106Social sciences2
 TOTAL16

 

YEAR THREE: FIRST SEMESTER

CODECOURSE  TITLECREDIT UNIT
ICH 201General Organic Chemistry2
ICH 211General Inorganic Chemistry2
ICH 221General physical chemistry2
BIO 251General Genetics3
BCH 351Biochemical Lab. techniques2
 TOTAL11

 

 

YEAR THREE: SECOND SEMESTER

CODECOURSE  TITLECREDIT UNIT
ICH 202General organic chem. II2
BCH 202Introduction to Met. Pathways2
BCH 222Bioenergetics and Biocatalysis2
BCH 232Physiological chemistry2
AMB 111Introductory Microbiology3
BCH 212Biological macromolecules2
 TOTAL13

 

 

YEAR FOUR: FIRST SEMESTER

CODECOURSE  TITLECREDIT UNIT
ICH 301Organic Chemistry2
BCH 301Primary metabolic pathways I3
BCH 311Cell and molecular biology3
BCH 321Introductory pharmacology2
BCH 331Biochem. Instru. and techniques3
BCH 341Food and nutritional biochem.2
 TOTAL15

 

YEAR  FOUR: SECOND SEMESTER

CODECOURSE  TITLECREDIT UNIT
BCH 242Computational biochemistry3
BCH 422Advanced clinical biochemistry2
BCH 432Immunochemistry and immunology3
BCH 452Biochemical pharmacology3
 TOTAL11

 

 

YEAR FIVE: FIRST SEMESTER

CODECOURSE  TITLECREDIT UNIT
BCH 361Plant Biochemistry3
BCH 421Biological memb. And bio sig.3
BCH 401Primary metabolic pathways II3
BCH 461Advanced biochemical methods3
 TOTAL12

 

YEAR FIVE: SECOND SEMESTER

CODECOURSE  TITLECREDIT UNIT
BCH 399SIWES6
 TOTAL6

 

 

YEAR SIX: FIRST SEMESTER

CODECOURSE  TITLECREDIT UNIT
BCH 411Frontiers in Biochem, Mol Biol and Biotech2
BCH 431Biochemistry of parasites2
BCH 441Biotechnology and industrial biochemistry2
BCH 451Microbial biochemistry2
BCH 499Research project2
 TOTAL10

 

 

YEAR SIX: SECOND SEMESTER

CODECOURSE  TITLECREDIT UNIT
BCH 402Advanced enzymology and reg. mechanisms3
BCH 412Biochemical reasoning and special topics2
BCH 442Molecular biology and genetic eng.3
BCH 499Research project2
 TOTAL10

 

COURSE DESCRIPTION APPLIED BIOCHEMISTRY

 

GSS 101   1 UNITS

See …………..

 

GS 105: Humanities (2 credit hours)

See ……………

 

GS 107 Nigerian Peoples and Culture (2 credit hours)

See ………………

 

MAT. 101 Elementary Mathematics 1 (3 credits)

Number systems- natural, integer, rational, irrational, real complex numbers. Elementary set theory. Indices, surds and logarithms. Quadratic equations. Polynomials and the factorizations: – the remainder and factor theorems. Rational functions and partial fractions. The PMI (Principle Mathematics Induction). PERUTATIONA AND COMBINATION. The Binomial theorem for rational index. Progression – arithmetic, geometric, harmonic, arithmetic – geometric. Solution of inequalities. The algebra of complex numbers: – addition, subtraction, multiplication and divisions: Argand diagrams and the geometry of complex number: Modulus; arguments and polar coordinates; the demoivre’s theorem. Complex nth roots of unity and complex solutions to Z= a. trigonometry- circular measures, addition, formulae and other trigonometric identities, sine and cosine laws; solutions of triangles, heights and distances.

 

CSC 101: Introduction to Computer Programming (3 Units)

History of computers; functional components of computer characteristics of a computer. Number systems, Boolean Algebra, Flowchart; Algorithms; statement symbolic names lists and arrays; subscripts, expressions and control statements in computer programming. Programming in BASIC. Computer application. Strategy for computer programming. Rules that guide the writing of BASIC programs/statements. Library functions. User-defined functions. Subprogram & Subprograms & Subroutine in basic.

 

PHY 101 General Physics 1 (3 Credits)

Space and Time, frames of reference, units and dimension, kinematics-vectors, scalars, speed/velocity, acceleration, circular motion and applications. Fundamental Laws of mechanics.

Statics: Equilibrium, Centre of mass,

Dynamics: Newton’s Law of Motion, Force, Inertia, Mass and weight, Contact forces, Atwood machine, Pulleys. Projectile motion.

Linear momentum. Galilean Invariance, Universal, Gravitation- Newton’s Gravitation

Law: Kepler’s Laws, Gravitational Potential, Earth’s Satellite, Velocity of escape and weightlessness. Work and energy. Rotational dynamics and Angular momentum, moment of inertia, Kinetic Energy of rotation; Conservation Laws, Oscillatory motion – Simple Harmonic, Damped and forced Oscillation.

 

ICH 101: Basic Organic Chemistry (2 Units)

Introduction

  • Brief Historical background, binding in organic compounds the carbon atom, hybridization ( SP3 SP2 SP).
  • Classification of organic compounds
  • Isolation and purification of organic compounds
  • Elemental or qualitative analysis
  • Quantitative analysis
  • Formulae of organic compounds- (empirical, molecular and structural). Structural and stereoisomerisms, Functional group and homologous series.

 

IUPAC Nomenclature

IUPAC  Nomenclature of the following family of organic compound: alkanes, alkenes, alkynes, halogenoalkanes, alkanols, alkanals, alkanones, alkanotes, acid amides, nitriles, and amines alkanoic acids, acid chlorides and acid anhydrides.

Preparation, physical and chemical properties of the families listed above.

 

ICH 111- General Basic Inorganic Chemistry I (2units)

Matter; Laws of Chemical Combination, Stoichmetry of chemical kinetics.

Atomic structure and electronic configuration of elements. Electronic theory of valences. Bonding; ionic, covalent, co-ordinate, metallic, hydrogen bonding and Van der Waals forces. Fundamental compounds of atoms stable and unstable particles, periodic classification of elements. Block, rows and groups. General features of chemistry of s-,p-,d and f-blocks isotopes detection, concentration and separation of isotopes.

 

BIO 101- General Biology I (3Credits)

Cell structure and organization of cellular organelles diversity characteristics and classification of living things, general reproduction, inter relationship of organisms, heredity and evolution, elements of ecology and types of habitats

 

GSS 102   1 CREDIT UNIT

See ………………

 

GS 106 Social Science (2 credit hours)

See …………………..

 

MAT. 102 Elementary Mathematics II (3Credits)

Functions: Concept and definitions, examples-polynomial, exponential logarithmic and trigonometric functions. Graphs and their properties. Plane analytic geometry:- equation of a straight line, circle, parabola, ellipse and hyperbola. Tangents and normal. Differentiation from first principles of some polynomial and trigonometric functions. Techniques of differentiation- sum, product, quotient and chain rule including implicit differentiation. Differentiation of simple algebraic, trigonometric, exponential, logarithmic and composite functions. Higher order derivaties. Applications to extramum and simple rate problems. L’ Hospital’s rule, simple Taylor/McClaurin expansion. Curve sketching. Applications to areas and volumes.

 

PHY 102 General Physics II (3Credits)

Electrostatic: Coulomb’s law, Gauss’s law, capacitors, Electric fields and potentials, energy in electric field. Conductors and currents; Ohms law, Temperature dependence of resistance combinations of Resistances. Measurement of Resistances, emfs. Dielectrics (Qualitative treatment only), magnetic fields and induction, faraday’s and Lewnz’s Laws, Earth’s field, ampere’s law. Maxwell’s equations (Qualitative treatment only),Electromagnetic oscillations and waves, types, properties. Mirrors and lenses, reflection, refraction, applications. Optical instrument.

 

 

ICH 102: Basic Physical Chemistry (2units)

The gas laws; Boyle’s law, Charles law, the general gas equation, Gay-Lussac’s Law; Gas constant. Dalton’s law of partial pressures, Graham’s law of gaseous diffusion. The kinetic theory of matter. Derivation of the kinetic equation Derivations from the ideal gas law; Vander waal’s equation, liquefaction of gases, the Joule-Thompson effect.

Properties of dilute solution

Definition  of the following  concentration terms; molarity, molality, mole fraction, vapour pressure and lowering of vapour pressure; Raoults law; elevation of boiling point, lowering of freezing point; osmotic pressure.

Introduction to Thermodynamics and thermo chemistry

Definition of the following terms: systems, state variables, and equilibrium, Isothermal and adiabatic processes, the first law of thermodynamics; work done for state changes (PV type)

 

Thermo chemistry: Heat changes, heat reaction, Laws of thermo chemistry (Lavoisier and Laplace and Hess law of constant heat summation). Application of the laws in calculations, Bond Energy.

Chemical Equilibrium:

Chemical equilibrium and reversible reaction. The law of mass Action; equilibrium constant, Factors affecting chemical equilibrium, Equilibrium between ions in the solid and liquid phases; solubility product, effect of a common ion on solubility product.

Chemical Kinetics

Rate of a chemical reaction; Factors affecting the rate of a chemical reaction.

Activation energy.

Ionic Equilibrium

Electrolytes; Acids and Bases: Bronzed Lowry, Lewis and Arhenius theories of acids and bases. PH and Dissociation constant, ionization of water Hydrolysis, Buffer solution and Buffer capacity.

 

CSC 102: Introduction to computer programming (3 Units)

Introduction to problem solving methods and algorithm development; designing  coding debugging and documenting programs (i.e. flow charts control statements INPUT/OUTPUT statements, DO statements, Array, Subscripts, Dimension statements) using techniques of FORTRAN.

 

BIO 102- General Biology II (3 Credits)

Generalized survey of plant and animal kingdoms based mainly study of similarities and differences in the external features and ecological adaptation of these forms.

 

BCH 102: INTRODUCTORY BIOCHEMISTRY (3 CREDITS)

S/NTOPICSDURATION (WEEKS)
1Historical perspectives of Biochemistry2
2The building units of macromolecules – structures and functions of amino acids, monosaccharides, glycerol, fatty acids, choline and nitrogenous bases. Abiotic synthesis of building units.2
3The classification and hierarchical organization of macromolecules.  Proteins – globular, fibrous, albumin, prolamin, gliadin, globulin and lignin. Lipids – phospholipids, sphingolipids, plasmologens, VLDL, HDLP, soap, micelles, membrane, e.t.c Nucleic acids – nucleosides, nucleotides, DNA, RNA, rRNA, tRNA, mRNA.3
4The physical and chemical properties of water, weak acids and bases, pH, pOH, pKa, pKb, etc. Buffer solutions. Preparation of solutions.2
5The properties and classification of enzymes1
6The living cells of plants and animals – Biochemical structures, size dimension and functions of organelles.2

 

ICH 112: Basic Chemistry (2Units)

Theory and practice of volumetric and qualitative inorganic analysis. Preparation of standard solutions, calculation of molarity and concentrations.

Method of dilution of solutions and calculation. Redox titration and calculations involved. Test for common anions and identification of SO42- , SO32-   , NO3 ,CO3  2-  , Cl, Br, I, NO2–    . Test for common cations: Fe2+, Fe3+, NH4+, Zn2+, Pb2+, Al3+, alkali and alkaline earth metals. Test for common ions in the first transition series e.g. Mn, Cr, Ni, Cu, etc. Group separation of cations.

 

MAT 201: Linear algebra I (3 Credit UNIT)

Vectors and vector algebra. Vector space over the real field.

Linear dependence and independence; basis and dimensions. The dot and cross products in 3- dimensions.  Equations of lines and plane in free space. Linear transformation and their representation by matrices. Matrix algebra. Operation on representation by matrices: – rank, range, null space, nullity. Determinants and inverses of matrices. Singular and Non-singular transformations.

 

PHY 201; General Physics (3Credits)

(Elementary Modern Physics)

Relativity; Inertial frames, Galilean transformations, Coriolis force, Defects in Newtonian Mechanics, the speed of light; the Lorentz transformation; transformation of velocities and acceleration. Length contraction and time-dilation. Mass Energy relation. Experimental basis of quantum theory: Blackbody radiation; electrons and quanta; Thomson’s and Rutherford’s models. Bohr’s theory of atomic structure; De Broglie hypothesis, the uncertainty principles; Schrodinger’s equation and simple applications. Radioactivity, X-rays and its applications.

 

ICH 211: General Inorganic Chemistry (2 Units)   

Detailed treatment of factors that constitute periodic properties. Ionization energy, electron affinity, electronagativity, chemical bonding: ionic, covalent, metallic bonds; van der waal’s forces; the hydrogen bond. Stoichiometric and non-stoichiometric defects. Introduction to molecular orbital and valence bond theories, molecular geometry and hybridization. Ionic Lattice, lattice energies, the Mandelung constant.

 

ICH 201: General Organic Chemistry (2 Units)

  1. Electron Effects in Organic molecules.

Electron displacement in molecules, inductive effects; electromeric effect, mesomeric effect and resonance;inductomeric effect hyperconjugation mesomerism, tautomerism.

  1. Introduction to reaction mechanism.

Breaking of covalent bonds, collision theory of reaction rates, molecularity of a reaction. Reaction kinetics, Order and molecularity.

  1. Preparation, physical and chemical properties and uses of various classes of organic compound such as alkanes, alkenes, alkynes,halogenoalkenes, alkanals, and alkanones, alkanoic acids. Derivative of alkanoic acids (namely acid chlorides, acid anhydrides, alkanotes and acid amides) Nitriles and amines.    A mechanistic approach should be used to explain the chemical reaction treated in each case.

 

ICH 221: General Physical Chemistry (2 Units)

Kinetic Theory: Behaviour of gases and kinetic theory. Ideal and non-ideal behavour of gases. Derivation of the kinetic theory equation for the  pressure of an ideal gas and deductions there from. Molecular and Collision-Collision diameter and number. Mean free path; viscosity of a gas; Boltzmann distribution law. Types of speed; mean velocity, root-mean-square velocity and most probable velocity.

Chemical Thermodynamics; Nature and scope of thermodynamics, important definitions, the first law of thermodynamics, calorimetry, standars molar enthalpies of formation and the determination of standard molar enthalpy for chemical reactions from tables of these; Heat capacities. The carnot Cycle.

 

Bio 251- General Genetics (3 Credits)

Historical background and mendel’s work. Basic terminologies, chemical nature of the gene and gene interactions. Hereditable and non- Hereditable characteristics. Sex determination, inheritance in population, multiple allels sex linkage, mutation; molecular basis of inheritance (DNA, RNA and protein synthesis).  The concept and theory of evolution, variation and natural selection. Aspects of human  genetics and common genetic diseases.

 

AMB 211: Introductory Microbiology and Brewing

Microbial cell types- Prokaryotes and Eukaryotes; bacterial cell structure and function. The biology of bacteria, fungi, protozoa, algae and viruses. Ecological relationship and the role of microorganisms in natural and special environment such as soil, water, milk, food industry and in diseases. Definition and scope of brewing science, interrelationship with other sciences, principles and application of brewing science dealing with beer production, its supply, quality and safety. Physiology and biochemistry of cereals used in dealing with beer production, its supply, quality and safety.  Physiology and biochemistry of cereals used in brewing barley; sorghum, maize, rice, millet, hops. Career opportunities for brewing scientists.

 

BCH 201: INTRODUCTORY BIOMOLECULAR CHEMISTRY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1The chemical composition of living organisms. The Geometry, Bond Strength, Electronegativity, Bond energies, Van der waals forces, radii or carbon, nitrogen, oxygen and sulphur bondings2
2The three dimensional structures of simple biomolecules, configuration and conformation, molecular model of small molecules, stereoisomerism and stereo specificity.2
3Molecular Model of small molecules, stereoisomerism, reactions and importance1
4Carbohydrate chemistry – classification, structure, isomerism, reactions and importance.2
5Lipid chemistry – classifications, structures and reactions.2
6Amino acids chemistry – classification, structures and reactions.2

 

BUS 101- Introduction to Business I (3 Credits)

A study of the nature of business organizations. The character of business from social and economic perspective. Scope of business; roles of business; external properties of the business; role of business; type of economic systems. Why study business.

 

FORMS OF BUSINESS OWNERSHIP;

Sole proprietorship, partnership, joint stock company, statutory corporations/public enterprises, and cooperative societies.

 

MANAGEMENT AND ORGANIZATION

Nature of management, functions of management, line and staff functions in organizations.

 

PRODUCTION AND MARKETING

Types of production; production processes; the marketing concept; marketing functions

 

THE FINANCE FUNCTION

Type of financing-short term, medium term, long term financing. Factors influencing choice of funds. Types of shares

 

THE ACCOUNTING FUNCTION

Purpose of accounting, principles of accounting, double entry bookkeeping, day books, Ledger accounts, the trial balance, profit and loss account, and the balance sheet.

 

MAT 202: Elementary Differential Equations (3 Credits)

Methods of integration, introduction to differential equations- classification, order, degree. Ordinary differential equations of the first order. Examples to illustrate the sources of differential equation from the physical and biological sciences-growth, decay, cooling problems and the law of mass action. Linear differential equation of second order. Application of the first and second order linear differential equations to falling problems and simple circuits. Laplace transformations.

 

STAT 202: Statistics for Applied Science (3 Credits)

Frequency distribution. Measures of tendency and measures of dispersion. Laws of probability. Sampling techniques. Probability distributions. The Binomial, Poisson and Normal distributions. Estimation and test of Hypothesis: z, t,x2 and F- tests, Regression and Correlation. Analysis of Variance. Contingency tables. Simple non-parametric tests.

 

 

ICH 202: General Organic Chemistry II (2 units)

Alicyclic hydrocarbons

Preparation proterties and uses of cycloalkenes, cycloalkanes, cycloalkadienes.

Aromatic hydrocarbon, aromaticity, properties of aromatic compound, chemical properties of benzene.electrophic substitution reaction, detailed mechanisms of the following electrophilic substitution reaction should be studied using benzene as example: halogenations, nitration, sulphonation, akylation, acylation, addition and hydrogenation.

Effect of substitutions on the reactivity and orientation of electrophic substitution of benzene. Ortho-, para, and meta directing groups. Reactions of the side chains of alkylbenzenes: ring substitution reactions, addition reactions, and side chain substitution reactions; oxidation of the side chain.

Polynuclear Compounds

Structure, synthesis and reactions of isolated ring systems (diphenyl, diphenylmenthane, diphenylstilbene) structure, synthesis and reactions of naphthalene, anthracene and phenanthrene.

 

BCH 202: INTRODUCTORY METABOLIC PATHWAYS (2 CREDITS)

S/NTOPICSDURATION (WEEKS)
1Spontaneous reaction, free energy change of reaction and activation energy.1
2The properties, nomenclature and function of enzymes1
3Glycolysis, gluconeogenesis and pentose phosphate pathways2
4Tricarboxylic acid cycle and oxidative phosphorylation2
5Beta-oxidation and starvation (Ketone bodies).1
6Amino acid pool, nitrogen balance, protein degradation, deamination and transamination reactions. The urea cycle.2
7Photosynthesis: The Light and dark reactions – Calvin cycle.2

 

 

BCH 212: BIOLOGICAL MACROMOLECULES (3 CREDITS)

S/NTOPICSDURATION (WEEKS)
1Carbohydrates and glycobiology – structure, activity and function.

Polysaccharides – starch, glycogen, cellulose, chitin and inulin. Proteoglycans, glycosaminoglycans, peptidoglycans.

Glycoproteins – lectins, selectins.

Glycolipids – gangliosides, lipopolysaccharide. Analyses of carbohydrates.

3
2Protein three-dimensional structure and activity – primary structure and protein sequence determination.

Secondary structures – peptide bond angle, length, rigidity and structure, Alpha-helix, Ramachandran plot, Beta-conformation, Beta-turns.

Tertiary structures – Fibrous proteins, alpha-Keratin, collagen, fibrin, Globular proteins-three dimensional structures, troponin, antibodies, myoglobin, ribonuclease, Lysozyme. Super-secondary structures.

Quaternary structures – multimeric proteins, rotational and helical symmetry. Haemoglobin, poliovirus coal protein, molecular size of proteins and limitation to size

3
3The use of X-ray and NMR for studying protein structures, protein folding and denaturation – -chaperones and chaperonins.1
4Nucleic acids and Nucleotides. Properties of Nucleotides, Absorption spectra, Tautomerism, phosphodiester bonds in Nucleic acids, base pairing and hydrogen bonding.1
5Three-dimensional structures of RNA – tRNA, rRNA, mono and polycistronic mRNA.

DNA-double helix, distinctive composition, A, B and Z forms. Triplex and Tetraplex DNA, H-DNA, hairpins and cruciforms.

2
6Non-enzymatic transformation of nucleic acids – pyrimidine dimerization, nucleic acid hybridization, denaturation of DNA. Functions of nucleotides – energy compounds, enzyme cofactors.2
7Lipids – Glycerophospholipids, sphingolipids, gangliosides, lipoproteins 

BCH 222: BIOENERGETICS AND BIOCATALYSIS (2 CREDITS)

S/NTOPICSDURATION (WEEKS)
1The laws of thermodynamics. Concept of free energy, entropy, high-energy compounds, free energy change for the hydrolysis of ATP, thiolesters and high-energy compounds.2
2Oxidation-reduction reaction, reduction potential, standard reduction potential and its measurement. Electron carriers.2
3Catalysis and activation energy – transition state reactions, Arrhenius equation, calculation of activation energy and energy of denaturation. Effect of temperature on catalysis.2
4Kinetics of enzyme catalyzed reactions. Michealis-Mention equation, Lineweaver-Burke plot and other transformations. Effect of pH and inhibitors.3
5Allosteric and regulatory enzymes.1
6Purification and quantitative estimation of enzymes activity. Purification tables and units of enzyme activity.2

    

BCH 232: PHYSIOLOGICAL CHEMISTRY (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Body fluids-classification and composition1
2Blood clotting, blood groups and rhesus factor. Homeostasis, regulation and disorders of acids-base balance. Homeostatic control of sugars2
3Renal systems- structure and functions1
4Nervous system-structure and function of neurons, the nerve impulse, organization of nervous system – peripheral and autonomic nervous system2
5Muscular system – types, structure and functions. Myocytes, actin, myosin and muscles contraction2
6Respiratory system – structure of the lungs, mechanism of breathing1
7Cardiovascular system – structure and function1
8Endocrine system – the pituitary, anterior and posterior lobe hormones1
9Gastrointestinal tract – digestion and absorption1
10Liver – structure and functions2

 

BCH 242: COMPUTATIONAL BIOCHEMISTRY AND MOLECULAR BIOLOGY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Introduction to the Internet – World wide web

Internet resources, browsers, search engines,

Pubmed, Databases and its retrieval

2
2Nucleotide Sequence databases and analysis. Genes and genome – prokaryotic and eukaryotic genes.

GenBank entry and Genome Databases, organelle genome. Gene identification with internet resources. Exploring the human genome

3
3Protein Sequence database – Sequence alignment and similarity search. Multiple sequence alignment.

Proteomics – analysis and internet resources.

ORFs. Introns, exons – Finding protein coding regions. Predicting protein structure-secondary structures and domains of proteins, structured classifications

3
4Phylogenetic analysis – Applications and inference. Evolution, Online and generic sources.1
5Biochemical data analysis and management. Spread sheet applications, Microsoft excel, Microsoft Access and SPSS, other simple and multiple regression and correlation software applications2

 

BUS 102- Introduction To business II (3Credits)

(Pre-requisite: BUS 101)

This course is designed to familiarize the student with forms of business and management process.

 

BUSINESS INTEGRATION AND COMBINATION

Types of business integration; reasons for business integration; problems of business integration.

 

GOVERNMENT/BUSINESS RELATIONS

Government and business; government in business; reasons for government involvement in business

 

BUSINESS ORGANIZATION AND SOCIAL RESPONSBILITY

What is social responsibility of business? Social issues facing business organizations; the debate on the social responsibility of business-Nigeria’s corporate experience.

 

INTERNATIONAL BUSINESS/INTERNATIONAL TRADE

Theories of international trade; reasons for international trade, terms of international trade.

 

MANAGEMENT OF INDUSTRIAL RELATIONS

Trade union formation and growth; Trade union objectives; problems of trade unions; formation of employer’s association; objectives and functions of employer’s association; the role of government in labour-management relatiionships

 

ICH 301: Mechanisms of organic reactions (2 units)

Basic concepts, brief review of orbital, hybridization and bonding. Classification of reagents and reactions. Investigations of reaction mechanism by Kinetic and non-Kinetic methods. Energy profiles and reaction rates. Application of above to various classes of reactions out listed below;

Displacement Reactions

Nucleophilic substitution at saturated carbonation: SN1 and SN2 mechanisms; effects of structure and solvent; stereochemical manifestations of the different mechanisms. Neighbouring group participation and molecular rearrangement. Electrophilic aromatic substitution; effects of substituent on reactivity and orientation of further substitution; ionic mechanism for nitration, halogenations and  sulphonation. Ortho para ratios.

Nucleophilic aromatic substitution: SN1 and SN1 benzene mechanisms, smiles rearrangement etc.

Elimination and Addition Reactions

Wand B elimination: E1 and E2 mechanisms. Saytzef and Hofmann rules; Dehydration and dehydrogenation. Addition to C-O, C-N and C-C multiple bonds.

 

Esterification and Hydrolysis

Mechanisms of acyl oxygen fissions and alkyl oxygen fissions. Tautomerism; Types and theories of tautomerism. Estimations of enol content in keto-enol tautomerism

 

BCH 301: PRIMARY METABOLIC PATHWAYS 1 (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Catabolism of glucose, galactose and fructose, etc., Glycolysis, regulation of glycolysis, Pasteur effect, anaerobic fermentation.2
2Gluconeogenesis-from lactate, amino acids, glycerol, Regulation of the Cori cycle.1
3The Citric Acid Cycle and the Glyoxylate Cycle1
4Electron Transport and Oxidative Phosphorylation, Mechanism of ATP synthesis, effect of ionophore, uncoupler and energy transfer inhibitors.2
5Glycogenesis and Glycogenolysis, regulation and synthesis of starch, sucrose and others.1
6The Pentose Phosphate Pathway1
7Lipid metabolism, dietary lipids and lipoproteins. Fatty acid oxidation, synthesis and degradation of ketone bodies. Synthesis of fatty acids, triacyglycerol, glycerophospholipids, sphingolipids, cholesterol and steroid hormones.3

 

 

BCH 311: CELL AND MOLECULAR BIOLOGY 1 (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1DNA structure and size, Chromatin and nucleoid structure, DNA supercoiling and conjugation2
2DNA metabolism – replication, repair and recombination1
3RNA metabolism – DNA transcription, reverse transcription, regulation of transcription, operons, post-transcriptional processing of RNA1
4Protein metabolism – protein synthesis, genetic code, initiation, elongation and termination of protein synthesis, post-translation processing, protein targeting and transport, protein degradation2
5Mutation, mutagenic agents and cancer1
6Molecular description of the life cycle of Lambda, M13, HIV, etc.1
6Organization of Prokaryotic and Eukaryotic genome, intron, exon, transposon, retrotransposon, GC, and AT content of genes, satellite DNA, palindrome, others1
7Molecular Tools – Restriction Endonucleases, DNA ligases, natural and artificial vectors of gene transfer, plasmids, cosmids, virus, Polymerase Chain Reaction, DNA hybridization, etc.2

 

BCH 321: INTRODUCTORY PHARMACOLOGY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Definition of common terms in pharmacology – drugs. Source and composition1
2Drug development/assays1
3Important Pharmaceutical preparations, drug identity and nomenclature.1
4Routes of drug administration, placebo effect, drug allergy, drug idiosyncrasy.1
5Drug interactions – cummulation, synergism, tolerance, antagonism1
6Drug tolerance and drug resistance1
7General pharmacologic response to drugs – nature and mechanism1
8Concept of agonists, antagonists and receptors1
9Quantitative pharmacology – dose response, therapeutic index (TI), lethal dose, etc.1
10Cellular metabolism in infected cells. Host parasite relationships1
11Biochemical basis of chemotherapy, Enzyme inhibitors as drugs1
12The physical basis of drug selectivity1
13Traditional medicine – management of malaria, sickle cell anemia, common cold, hepatitis etc. with plant extracts.1

 

BCH 331: BIOCHEMICAL INSTRUMENTATION AND TECHNIQUES (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Microscopy, Light and Electron microscopy, ion probe analysis and video microscopy1
2Calomel and Glass electrodes for pH measurement.1
3Ultra filtration and dialysis.1
4Centrifugation – principles, differential, density gradient, ultra centrifugation: analysis of subcellular fraction1
5Spectroscopy-spectrophotometry, turbidometry, nephrometry, Atomic absorption, atomic emission, spectroflourimetry2
6Chromatography – principles. Types – paper, thin layer, absorption, molecular exclusion, partition, affinity, ion exchange, gas-liquid, high performance, low pressure. Detection, quantification and analysis of biomolecules by chromatography2
7Electrophoresis cellulose acetate, agarose gel, starch gel, SDS- polyacrylamide gel, isoelectric focusing detection, estimation of molecular weight of protein. Western, Northern and Southern blotting2
8Radioisotopic techniques – Nature of radioactivity, counting, radioactivity and data analysis, ELISA, Application in immunology and bioassays2

 

BCH 341: FOOD AND NUTRITIONAL BIOCHEMISTRY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Food nutrients-carbohydrates, proteins, essential amino acids, fats, water.1
2Food nutrients-vitamins and their co-enzyme derivatives, A, B, C, D, E, K, Lipoic acid, pantothenic acid, folic acid, biotin, choline-structures, sources, reactions and deficiency syndrome of vitamins2
3Food nutrients requirement sources, reactions and deficiency syndrome of macro and micro minerals. Relationship between physiochemical and biological function of inorganic ions, ligand complexes and function1
4Food analysis proximate analysis, moisture and dry matter, organic and inorganic matter, ash1
5Basal Metabolic Rate (BMR), measurement and factors that influence BMR.2
6Anutrients in food-phytohaemagglutinin, protease inhibitors, phytate, phytoestrogen, sapponin, tannins, polyphenols, flatulence factors, amylase inhibitors, anti-vitamins, amino acid analogues, goitrogens, etc. Beneficial effects of anutrients2
7Food additive, food toxin: monosodium glutamate, artificial sweeteners, colouring, aflax1
8Nutritional disorders: protein energy malnutrition, obesity and diabetes1
9Nutritional status: recommended daily allowance.1
10Diet and physiological stress, ageing, pregnancy and athletics.1

 

BCH 351 BIOCHEMICAL LABORATORY TECHNIQUES (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Introduction- The work environment, health and safety. Analysis of data. Graphs, bar, histogram, pie-chart and rectangle co-ordinates, plot y vs x, standard curve, average, standard error, standard deviation, confidence limits, frequency, least square methods, etc.1
2Solutions – distilled water, de-ionized water, buffer solution – titration of weak acids and bases, calculation and preparation of standard solutions. Solutions of standard ionic strength1
3Carbohydrate – quantitative and qualitative analysis, Molisch, Bial’s Seliwanoff, iodine, Barfoed, Benedict and Fehling tests. Estimation of starch and glycogen content – Anthrone, Phenolsulphuric acid methods1
4Amino Acids- qualitative and quantitative analysis; Xanthoproteic, Millions, Sakaguchi and Ninhydrin reactions1
5Lipids-qualitative and quantitative analysis. Acid value of oils, saponification value, isolation of fatty acids.1
6Nucleic acids and Nucleotides – Isolation of RNA and DNA1
7Estimation of RNA and DNA tissue. Effect of temperature on the viscosity of DNA1
8Chromatography- paper, thin layer and adsorption. Separation of ink and plant pigments1
9Chromatography- Gel and ion exchange, separation of proteins.1
10Electrophoresis – paper and gel. Separation of haemoglobin A and S

Metabolisms:

·         Photosynthesis- isolation of chloroplast, reduction of DCPIP by chloroplast.

·         Blood glucose levels in diabetes

·         Metabolic changes associated with organisms.

·         Bioluminiscenses

1

BCH 361: PLANT BIOCHEMISTRY (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Organization of plant cells – structure of plant cell wall and function of organelles. Plant cell wall – three dimensional structure composition and function1
2Stages in plant development, totipotency, plant cell tissue and organ feature, seed germination and growth.1
3Photosynthesis – C3, C4 and crasulassean acid metabolism in plants photorespiration1
4Nitrogen metabolism – nitrogen cycle and non-protein amino acids. Transport and storage of amino acids in plants. Classification of plant proteins. Cyanogenic glycosides – structure and toxicity. Sulphur cycle1
5Plant root and soil interaction. Plant exudates, symbiotic and non-symbiotic nitrogen fixation1
6Phytohormones and related compounds: auxins, gibberellins, cytokinins, abscisic acid, ethylene, and other plant growth regulators1
7Herbicides – mechanism of action, residue determination and toxicity1
8Nitrogen compounds – classification, structure and biosynthesis of alkaloids, chlorophyll, model and amines1
9Plant phenolics – structure, function and biosynthesis of phenols, phenolic acids, phenylacetic acid, hydroxyciannamic acid, quinins, xanthones, stilbenes, flavonoids, lignans and neolignans, biflavonoids, tannins and melanins2
10Trepenoids – structure, synthesis and function of essential oils, diterpenoids, triterpenoids, steroids, carotenoids and polyisoprene.1
11Physiochemical methods – methods of plant analysis-extraction, separation, identification and analysis of plant component, fractionation into different class of polarity.1

 

YEAR THREE, SECOND SEMESTER COURSE

BCH 399: SIWES (6 CREDITS)

S/NOTOPICDURATION

(WEEKS)

 Students Industrial Work Experience Scheme (SIWES)6

 

BCH 401: PRIMARY METABOLIC PATHWAYS II (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Nitrogen metabolism, nitrogen cycle, nitrogen fixation, glutamine synthetase in nitrogen metabolism2
2Amino Acid biosynthesis and its regulation. Defects in amino acid metabolism. Synthesis of molecules derived from amino acids-porphyrins, creatinine, glutathione, amino acids, others.2
3Nucleotide and nuleic acid synthesis. Salvage and de-novo pathways and regulation2
4Nitrogenous excretory products – amino acid oxidation, production of urea, degradation of nucleotides, nuleic acid, purines and pyrimidines: Lesch-Nyhan syndrome, Gout.2
5Integration and hormonal regulation of mammalian metabolism. Tissue specific metabolism, hormonal regulation of such metabolism-epinephrine, glucagons, insulin, cortisol3

 

 

 

BCH 411: FRONTIERS IN BIOCHEMISTRY, MOLECULAR BIOLOGY AND BIOTECHNOLOGY (2 CREDITS)

 TOPICSDURATION

(WEEKS)

 Students are assigned topics that will expose them to current literature search and contemporary studies to be presented in weekly Department seminar. Presentation which must be prepared using PowerPoint presentation and abstract of the presentation should be submitted one week before actual presentation date.

Seminar grading scheme includes abstract, scope of content, standard of presentation, conclusion, references and response to questions

 

 

BCH 421: BIOLOGICAL MEMBRANE AND BIOSIGNALLING (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Structure, composition and function of biological membranes-lipids, proteins and carbohydrates. Biogenesis – isolation and characterization of membranes. Artificial membranes2
2Membrane dynamics. Phase transition, motion of membrane components, heterokaryon membrane fusion – exocytosis, endocytosis1
3Transport across membrane – simple and facilitated diffusion exchange diffusion, symport, uniport, active transport – free energy of transport, primary and secondary active transport systems. Classification of ATPases, ion gradient for transport, ion-selective channels, acetylcholine receptor, porins, etc., defective ion channel, ionophores2
4Signal transduction features

·         Gated ion channels – electrical signaling

·         Ligand-gated ion channel, nicotinic acetylcholine receptor.

·         Voltage-gated ion channel, neuronal action potential

2
5Receptor enzymes signal transductions – tyrosine-specific protein2
6Kinases, insulin-receptor protein kinase, G guanyl cyclase G-protein coupled receptors: beta-Adrenergic receptor – epinephrine

·         Camp signaling

·         Diacyglycerol and IP3 signaling.

·         Calcium signaling

2
7Sensory transduction in vision, olfaction and gustation1

 

 BCH 431: BIOCHEMISTRY OF PARASITES (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Review of major tropical pathogenic viruses and bacteria, tropical parasitic protozoa and metazoan2
2Distribution and metabolism of inorganic substances in tropical parasites. Disturbance of host inorganic metabolism. Metabolism in parasitic infections.2
3Distribution and metabolism of carbohydrates, proteins, lipids, nucleic acids and vitamins in tropical parasites, peculiarities of the metabolic machinery of parasites are highlighted.2
4Respiration – aerobic and anaerobic respiration in parasites: respiratory chain, oxidative phosphorylation, host-induced variations in respiration of the host2
5Control of parasitic infections – control of vectors, mechanism of action of insecticides, biological control of vectors, mechanism of parasitic diseases2
6Chemotherapy – mechanism, differences in the metabolism of carbohydrates, lipids, nucleic acids and proteins. Enzyme and organelle targets to be emphasized2

 

BCH 441: BIOTECHNOLOGY AND INDUSTRIAL BIOCHEMISTRY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Bioreactors – principles, designs and application, Plant and animal cell culture1
2Fermentation technology-fermentation products – alcoholic, amino acid and secondary metabolites. Antibiotic products of biotechnology – structures, vinegar, organic flavour enhancers, ogili2
3Strain selection and development – mutagenesis, over-production of metabolites and gene dosage. Screening and selection of micro-organism of industrial importance2
4Genetic Engineering for improved plants and animals, metabolic engineering, protein engineering and antibody engineering1
5Principles of industrial enzymology. Principle and application of cell and enzyme immobilization. Large-scale protein/enzyme purification techniques. Application of enzymes in industrial and clinical analysis. Biosensors.2
6Industrial pollution and bioremediation1
7Process evaluation, development, quality control and industrial standards.1
8Industrial experiences – invitation of industrialist for discussion of topic of interest; discussion of industrial attachment and experiences1

 

 

                   BCH 451: MICROBIAL BIOCHEMISTRY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Classification and structure of bacterial, fungal and protozoan cells2
2Nutritional categorization – autotroph, heterotroph, photolithotroph, photoorganotroph, chemolithotroph and chemooganotroph. Auxotrophic organisms – importance2
3Biosynthesis, structure and function of bacterial and fungal cell wall2
4Metabolic pathways unique to microorganism: phosphoketolase pathway2
5Entner – Duodorof pathway, modification of glycolytic and pentose phosphate pathways.1
6Antibiotics – synthesis and activities. Biochemical basis for antibacterial, antifungal and antiprotozoan action. Antiseptics and disinfections2

 

 

                 BCH 461: ADVANCED BIOCHEMICAL METHODS (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Exposure of students to operation of available laboratory equipment: Practicals and assignment1
2Preparation of dissertation/theses. Journal publication and review. Term paper presentation of mini-project in approved format.2
3Frontiers in Biochemical instrumentation – literature reading in new techniques and instrumentation1
4Spectroscopic techniques – principles, instrumentation, application of x-rays, gamma-ray resonance, infra-red and Raman spectroscopy, electron spin resonance, nuclear magnetic resonance, circular dichroism and luminometry.2
5Mass spectrometric techniques – principles, instrumentation and applications. Mass spectrometry, electron impact ionization, chemical ionization, field ionization, ion desorption methods, ion evaporation methods, analyzers, detectors and tandem mass spectroscopy.3
6Electrochemical techniques – principles, potentiometry, voltametry.2

 

 

BCH 402: ADVANCED ENZYMOLOGY AND REGULATORY MECHANISMS      (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Kinetics of ligand binding – Bonding of oxygen to haemoglobin and myglobin. Antigen-Antibody interaction. Hill’s equation, Scatchard plot1
2Multisubstrate Kinetics – sequential, ping-pong and other mechanisms1
3Inhibition studies – reversible and irreversible inhibition, substrate and product inhibition – competitive, non competitive, uncompetitive and mixed-type2
4Chemistry of enzyme catalysis, rate enhancement, binding energy, transition state stabilization, desolvation, induced fit, covalent catalysis, acid-base catalysis, proximity effect, metal ion catalysis1
5Effect of pH, Optimum pH and pKa value of active site residues, ionization of enzymes, enzyme-substrate complex1
6Effect of temperature – free energy changes, Equilibrium constant, Arrhenius equation, Vant Hoff’s equation, thermal denaturation of enzyme1
7Pre-steady state kinetics, instrumentation and techniques2
8Regulatory enzymes – molecular model for allosterism, committed step enzymes, feedback and feed forward inhibitors and cumulative feedback inhibition. Regulation by covalent modification and proteolytic cleavage1
9Mechanism of catalysis for chymotrypsin, phosphofructokinase, ribonuclease, etc.1
10Computational Enzymology-Expert Protein Analysis System (http://www.expasy.ch/enzyme)

Enzymology database (http:wrtpincs.anl.gov/EMP/)

BRENDA: (http://www.brenda.unikoeln.del).

Ligand database enzyme structure:

(http: www.biochem.ucl.ac.uk/bsm/enzymes/index-html)Analysis of enzyme kinetic data

1

 

                BCH 412: SPECIAL TOPICS IN BIOCHEMISTRY (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

 A reading list  is given to students at the beginning of the semester. An examination question includes 30%  BCH 411, Practical Manuals 25%, Computational Biochemistry 5%, Historical Perspective of Biochemistry 10%, Biochemical Reasoning 10% and Concepts in Biochemistry 10% objective question into 100 in number. 

 

 

BCH 422: ADVANCED CLINICAL BIOCHEMISTRY (3CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Biochemical tests and diagnosis – basis or uses, interpretation, factors affecting tests, variations, types of specimens for tests. Specimen preservation and handling1
2Diagnostic enzymes – causes of release of enzymes and plasma enzymes of clinical importance.1
3Liver disease – tests used to assess liver diseases, E.g., liver function tests, bilirubin production and metabolism. Jaundice types2
4Metabolic disorders – diabetes mellitus, diabetes insipidus, glycogen storage disease and obesity2
5Disorders of lipid metabolism – lipoprotein metabolism and diseases associated with lipid metabolism2
6Renal diseases – clearance tests, renal failure – pre-renal, intrinsic, acute, post-renal and chronic renal failures. Nephritic syndrome and causes. Electrolyte metabolismI
7Gastrointestinal tract diseases – stomach function tests, pentagastrin and gastrin tests. Pancreatic function tests – direct and indirect tests. Malabsorption – causes and diagnosis1
8Reproduction – male and female sex hormones, puberty, menopause, amenorrhea, hirsutism, pregnancy and infertility, contraceptives1
9Thyroid disorders – functions, tests and disorders1
10Biochemistry of Paediatrics and Geriatrics – prenatal diagnosis1

 

BCH 432: IMMUNOCHEMISTRY AND IMMUNOLOGY (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Innate and acquired immunity – features, mechanisms and determinants1
2Cells and organs of the immune system. Evolution of the immune system1
3Antigens – antigenic determinants, haptens, immunogens1
4Antibody structure and function – classification, polyspecific and monoclonal1
5Lymphocytes – isolation and function. Antigen-antibody interaction – characteristics, precipitation, lattice theory, agglutination and haemagglutination1
6Detection of antigen-antibody reaction – immunodiffusion, immunoelectrophoresis, fluorescence quenching, radioimmunoassay, enzyme-linked immunosorbent assay, fluorescent antibody technique3
7The Major Histocompatibility Complex (MHC), MHC restriction1
8Cytokines1
9The complement system and its activation. Hypersensitivities. Immunologic tolerance, autoimmunity and immunodeficiency2
10Transplantation1
11Immunology of parasitic diseases2

 

     BCH 442: MOLECULAR BIOLOGY AND GENETIC ENGINEERING (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Mutations, site directed mutagenesis, the environment and mutagenesis, Ames test, Cancers2
2Recombinant  DNA Technology – DNA library, cDNA library, methods of engineering new products2
3The cell cycle and cell death – mitosis, mitotic phases, apoptosis and meiosis, regulation of cell cycle, oncogenes and tumour suppressor genes2
4Molecular medicine – the human genome. Structural organization, proteomics, monogenic and polygenic disorders, pre-natal diagnosis, viral infections, gene therapy2
5Molecular motor proteins and functions. Structure of molecular motors and accessory proteins, formation of motile assemblies in cells. Functional properties of the biological machine2
6Mechanism of protein degradation. Ubiquitination and proteosome, autophagy. Proteolytic enzymes – specificities and functions2
7Frontiers in cell and molecular biology – current literature and discussions2

 

 BCH 452: BIOCHEMICAL PHARMACOLOGY (3 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

1Drug metabolism – phases, drug metabolizing enzymes and factors affecting metabolism3
2Drug distribution – bioavailability, drug reservoirs: plasma protein and tissue binding, redistribution, blood-brain barrier, foetal-placental barrier1
3Excretion – renal, biliary and faecal excretion. Excretion by other routes – sweat, saliva, breast milk1
4Pharmacokinetic parameter, kinetics of drug absorption and elimination, clearance, volume distribution, half-life and route bioavailability3
5Dosage regimens – maintenance, loading and individualizing doses. Factors that determine relationship between prescribed drug dosage and drug efficacy2
6Pharmacogenetics.2

 

 BCH: 499 UNDERGRADUATE RESEARCH PROJECT (2 CREDITS)

S/NOTOPICSDURATION

(WEEKS)

 Research problems involving laboratory work in an area of interest. Instructor/ Supervisors are assigned to students. Research is to be carried out in the first and second semesters of the final year15